Experimental: Wetlab Team

Clonebots Assembly

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Project Leads: Arvind Swamynathan, Cherry Wu
Members: Coming soon!

Traditional plasmid assembly methods (e.g. Golden Gate, Gibson) are performed in vitro, requiring many intermediate steps. Our team utilizes a CRISPR system to construct DNA parts in vivo, resulting in a faster and more efficient way to perform DNA assembly. We are presently optimizing double transformation of indicator and editor plasmids, as well as testing conjugation of pOX28 plasmids.

Check out our poster showcasing our most recent progress.